Shallot (Allium cepa L.) Skin Ethanol Extract Neutralizes Liver Oxidative Stress in Diazinon-Induced Wistar Rats

ABSTRACTDiazinon has a hepatotoxic effect since it is metabolized in the liver involving Kupffer cells (KCs) activation, increasing reactive oxygen species (ROS). The flavonoids contained shallot (Allium cepa L.) skin act as antioxidants neutralizing oxidative stress. This study aims to determine of ethanol extract (SSEE) on MDA level and activated KCs histopathology. total SSEE was measured using aluminum chloride colorimetric method resulted 228.1 mg QE/g. Rats were divided into normal, diazinon, groups. Diazinon administered at dose 40 mg/kg b.w. for 7 days, followed by 600, 900, 1,200 days. Liver malondialdehyde (MDA) MDA-TBA method. results revealed that diazinon increased (p <0.05), while doses 900 decreased equal normal (p>0.05). Activated group impressively group. In conclusion, neutralize stress due indicated decrease normal.Keywords: antioxidant, cell, liver, MDA, shallotCorrespondence: [email protected]